中國科學院院士

美國醫學與生物工程院（AIMBE）fellow

中國科學院過程工程研究所研究員

生化工程國家重點實驗室主任

中國顆粒學會副理事長

中國化學會會士

中國化工學會會士

中國微米納米技術學會會士

中國化工學會生物化工專業委員會副主任委員

中國生物工程學會生物基材料專業委員會主任委員

報告題目：新型分離介質的制備和蛋白藥/疫苗純化應用
Preparation of Novel Microspheres for Protein / Vaccine Separation Application
Polysaccharide particles such like agarose particles have been widely used in protein separation and purification as chromatographic packing materials by biological scientists and in industry. However, the limitation of conventional agarose particles is that the separation resolution is limited due to their broad size distribution. Furthermore, the particles with large size are usually used for industrial separation and purification to avoid the increase of back-pressure, which also limit the separation resolution. On the other hand, the particulate vaccine such like virus-like particle (VLP) has attracted more and more attention, conventional agarose particles with small pore size not only limited the adsorption of VLP, but also enhanced the disassociation of VLP, resulting very low activity recovery of VLP.We have developed a novel membrane process to prepare uniform agarose particle. By this technique we can control the CV (coefficient of variation) value which representing the size distribution around 15%, and we can prepare small uniform particles with high agarose concentration. Therefore, we can use small particles instead of large particles, and increase the separation resolution and flow rate of chromatography.  We have developed a new process to prepare giga-porous particle with pore size controllable between 100nm to 500 nm, and we found that it not only increased the loading amount of VLP, but also avoided the disassociation of VLP. This was because large pore size weakened the multi-site interaction between VLP and pore. This novel particle has been used for production of particulate vaccine instead of ultra-high speed centrifugation technique, much higher recovery was achieved.

個人簡介
馬光輝，中國科學院院士，美國醫學與生物工程院（AIMBE）fellow，生化工程國家重點實驗室主任。國家杰出青年獲得者，基金委創新群體首席，中國顆粒學會副理事長，中國化學會會士、中國化工學會會士、中國微米納米技術學會會士、中國化工學會生物化工專業委員會副主任委員、中國生物工程學會生物基材料專業委員會主任委員。研究方向為均一生物微球和微囊的制備及其在生化工程和醫學工程中的應用，研究和開發用于生化分離、藥物載體、免疫佐劑（疫苗遞送系統）、細胞培養微載體、酶固定化載體等創新產品。在Nat. Mater., Sci. Transl. Med., Nat. Nanotechnol., Nat. Biomed. Eng., Sci. Adv., Nat. Commun., JACS, Adv. Mater.等國際著名學術期刊上發表SCI論文500余篇。出版中英文專著12部，撰寫學術書章節22篇。中國發明專利授權81項，國際專利授權12項，專利技術和產品在國內外500多家單位得到應用。獲國家技術發明二等獎、北京市科學技術一等獎、中國化工學會科學技術獎基礎研究成果獎一等獎、中國顆粒學會自然科學獎一等獎、中國石油和化學工業聯合會技術發明一等獎、侯德榜化工科技創新獎、“中國科協全國優秀科技工作者”稱號。